| 1. Identity statement | |
| Reference Type | Journal Article |
| Site | mtc-m16c.sid.inpe.br |
| Holder Code | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S |
| Identifier | 8JMKD3MGP8W/34A6RLS |
| Repository | sid.inpe.br/mtc-m18@80/2008/11.26.13.04 (restricted access) |
| Last Update | 2009:01.06.19.26.14 (UTC) administrator |
| Metadata Repository | sid.inpe.br/mtc-m18@80/2008/11.26.13.04.33 |
| Metadata Last Update | 2018:06.04.04.05.47 (UTC) administrator |
| Secondary Key | INPE--PRE/ |
| DOI | 10.1039/b810085c |
| ISSN | 1474-905X |
| Citation Key | SchuchGalLimSchMen:2008:DeDNSy |
| Title | Development of a DNA-dosimeter system for monitoring the effects of solar-ultraviolet radiation  |
| Year | 2008 |
| Month | November, 20th |
| Access Date | 2024, May 18 |
| Secondary Type | PRE PI |
| Number of Files | 1 |
| Size | 443 KiB |
| 2. Context | |
| Author | 1 Schuch, André Passaglia 2 Galhardo, Rodrigo da Silva 3 Lima-Bessa, Keronninn Moreno de 4 Schuch, Nelson Jorge 5 Menck, Carlos Frederico Martins |
| Resume Identifier | 1 2 3 4 8JMKD3MGP5W/3C9JHUD |
| Group | 1 2 3 4 RSU-CIE-INPE-MCT-BR |
| Affiliation | 1 Department of Microbiology, Isntitute of Biomedical Sciences, University of São Paulo 2 3 4 Instituto Nacional de Pesquisas Espaciais (INPE) |
| Journal | Photochemical & Photobiological Sciences |
| Volume | V. 8 |
| Number | pg. 111 - 120 |
| Pages | http://www.rsc.org/delivery/_ArticleLinking/ArticleLinking.cfm?JournalCode=PP&Year=2009&ManuscriptID=b810085c&Iss=Advance_Article |
| History (UTC) | 2009-01-07 15:55:02 :: seeger05 -> administrator :: 2010-05-11 01:25:11 :: administrator -> seeger05 :: 2010-05-11 16:15:38 :: seeger05 -> administrator :: 2018-06-04 04:05:47 :: administrator -> seeger05 :: 2008 |
| 3. Content and structure | |
| Is the master or a copy? | is the master |
| Content Stage | completed |
| Transferable | 1 |
| Content Type | External Contribution |
| Abstract | Solar radiation sustains and affects all life forms on Earth. In recent years, the increase in environmental levels of solar-UV radiation due to depletion of the stratospheric ozone layer, as a result of anthropogenic emission of destructive chemicals, has highlighted serious issues of social concern. This becomes still more dramatic in tropical and subtropical regions, where the intensity of solar radiation is higher. To better understand the impact of the harmful effects of solar-UV radiation on the DNA molecule, we developed a reliable biological monitoring system based on the exposure of plasmid DNA to artificial UV lamps and sunlight. The determination and quantification of different types of UV photoproducts were performed through the use of specific DNA repair enzymes and antibodies. As expected, a significant number of CPDs and 6-4PPs was observed when the DNA-dosimeter system was exposed to increasing doses of UVB radiation. Moreover, CPDs could also be clearly detected in plasmid DNA when this system was exposed to either UVA or directly to sunlight. Interestingly, although less abundant, 6-4PPs and oxidative DNA damage were also generated after exposure to both UVA and sunlight. These results confirm the genotoxic potential of sunlight, reveal that UVA may also produce CPDs and 6-4PPs directly in naked DNA and demonstrate the applicability of a DNA-dosimeter system for monitoring the biological effects of solar-UV radiation. |
| Area | CEA |
| Arrangement | urlib.net > BDMCI > Fonds > Produção anterior à 2021 > CRCRS > Development of a... |
| doc Directory Content | access |
| source Directory Content | there are no files |
| agreement Directory Content | there are no files |
| 4. Conditions of access and use | |
| Language | en |
| Target File | Development of a DNA-dosimeter system for monitoring ...b810085c André P. Schuch.pdf |
| User Group | administrator seeger05 |
| Visibility | shown |
| Copy Holder | SID/SCD |
| Archiving Policy | denypublisher denyfinaldraft12 |
| Read Permission | deny from all and allow from 150.163 |
| 5. Allied materials | |
| Next Higher Units | 8JMKD3MGPCW/3EUFCFP |
| URL (untrusted data) | http://xlink.rsc.org/?doi=B810085C |
| Dissemination | WEBSCI; PORTALCAPES. |
| Host Collection | sid.inpe.br/mtc-m18@80/2008/03.17.15.17 |
| 6. Notes | |
| Empty Fields | alternatejournal archivist callnumber copyright creatorhistory descriptionlevel documentstage e-mailaddress electronicmailaddress format isbn keywords label lineage mark mirrorrepository nextedition notes orcid parameterlist parentrepositories previousedition previouslowerunit progress project readergroup rightsholder schedulinginformation secondarydate secondarymark session shorttitle sponsor subject tertiarymark tertiarytype typeofwork versiontype |
| 7. Description control | |
| e-Mail (login) | seeger05 |
| update | |